RSC Publishing


Publishing

 

Cover image for Physical Chemistry Chemical Physics, select for current issue

Physical Chemistry Chemical Physics

High quality research in physical chemistry, chemical physics and biophysical chemistry.



Subscribers

Non-subscribers

Free access



Paper

Phys. Chem. Chem. Phys., 2009, 11, 10466 - 10472, DOI: 10.1039/b912684h

Hydration structures of 2-deoxyguanosine studied by IR-UV double resonance spectroscopy: comparison with guanosine

Hiroya Asami, Shu-hei Urashima and Hiroyuki Saigusa

Infrared spectra of mono- and dihydrated clusters of 2-deoxyguanosine (2-dGs), which are formed by laser desorption combined with supersonic jet-cooling, have been measured by the technique of IR-UV double resonance spectroscopy. The structures of these hydrates are compared with those reported for guanosine (Gs) to elucidate the importance of the 2-hydroxy group in the hydration. It is shown that monohydrated structures observed for 2-dGs are similar to those of Gs, indicating no significant influence by the absence of 2-OH group. For the dihydrated cluster, two structural isomers are identified and assigned to the dihydrates with the guanine moiety is in the keto form, which are consistent with the lowest-energy structures derived from theoretical calculations. Comparison with the results for Gs suggests that the presence of 2-OH group leads to the stabilization of specific dihydrate structures involving the sugar group.

Graphical abstract image for this article (ID: b912684h)