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Paper
J. Anal. At. Spectrom., 2007, 22, 1061 - 1066, DOI: 10.1039/b704807f
Application of species-specific isotope dilution analysis to the correction for selenomethionine oxidation in Se-enriched yeast sample extracts during storage
Zoyne Pedrero, Jorge Ruiz Encinar, Yolanda Madrid and Carmen Cámara
This paper reports the advantages of isotope dilution analysis (IDA) in the correction for selenomethionine oxidation in Se-enriched yeast reference material extracts. Enzymatic hydrolysis was performed under two different conditions: by using ultrasound probe sonication for 2 min; and incubating at 37 °C for 24 h. [76Se]SeMet isotopically labelled spike was added to the sample after Se species extraction, and 80Se/76Se and 78Se/76Se isotope ratios were measured as peak area ratios after HPLC-ICP-MS. The stability of the extracts obtained with both hydrolysis methods were evaluated by comparison of results of those immediately analysed with those stored for 24 h at different temperatures. The selenomethionine oxidation process showed a strong dependence on the extraction method used and the storage temperature, leading to biased selenomethionine content determinations. Species-specific isotope dilution analysis was successfully applied for the correction for such selenomethionine oxidation during storage.
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