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Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering
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Paper
Lab Chip, 2009, 9, 3261 - 3267, DOI: 10.1039/b907724c
On-chip immuno-agglutination assay with analyte capture by dynamic manipulation of superparamagnetic beads
Y. Moser, T. Lehnert and M. A. M. Gijs
Magnetic bead-based lab-on-a-chip systems offer significant advantages compared to more conventional systems, mainly through the possibility of controlled manipulation of the magnetic carriers on-chip. In particular, microfluidic immunoassays using functionalized magnetic beads raise increasing interest. We present here a new approach for performing immuno-agglutination assays on-chip. Our system is based on a quadrupolar magnetic field set-up. Dynamic actuation of a confined plug of functionalized magnetic beads is used for analyte capture in a microchannel. A simple detection method based on the swelling of the released plug after agglutination is presented. We demonstrate the feasibility of on-chip agglutination tests by means of a streptavidin/biotinylated-bovine serum albumin (bBSA) model assay. A detection limit of about 200 pg/mL (
3 pM) is achieved.
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