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Molecular BioSystems

Research at the interface between chemistry and the -omic sciences and systems biology.



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Review

Mol. BioSyst., 2008, 4, 481 - 495, DOI: 10.1039/b801666f

Enabling methodology for the end functionalisation of glycosaminoglycan oligosaccharides

Emiliano Gemma, Odile Meyer, Duan Uhrín and Alison N. Hulme

The chemical functionalisation of glycosaminoglycans is very challenging due to their structural heterogeneity and polyanionic character; but as an enabling technology it promises rich rewards in terms of the structural and biological data it will afford. This review surveys the known methods for the preparation of glycosaminoglycan oligosaccharides and conditions for the selective functionalisation of both the reducing and non-reducing ends. The synthetic merits of each approach are discussed, together with the structural modification of the glycosaminoglycan oligosaccharide which they confer. Recent applications of this methodology are highlighted, including introduction of functional labels for gel mobility shift assays and NMR studies of glycosaminoglycan–protein complexes, and synthesis of immobilised glycosaminoglycan arrays.

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