_tcm18-11405.gif )
Latest News
Highlights in Chemical Science issue 12 now online
24 November 2009
Drawing together the best content from all RSC publications, to provide a snapshot of the latest developments across the chemical sciences.
Making microfluidic membranes
18 November 2009
US scientists have made biocompatible membranes in microfluidic chips
Lab on a Chip Newsletter
The Lab on a Chip newsletter "Miniaturisation Report" is full of news and insights, enabling you to easily stay up to date with current developments. Sign up for Miniaturisation Re...
Lab on a Chip video channel
Find out more about Lab on a Chip videos
Contents list for Lab on a Chip, issue 24, 2009
Front cover
Lab Chip, 2009, 9, 3453
DOI: 10.1039/b923621j
Inside front cover
Lab Chip, 2009, 9, 3454
DOI: 10.1039/b923623f
Contents and Highlights in Chemical Technology
Lab Chip, 2009, 9, 3455
DOI: 10.1039/b923624b
Highlight
Research Highlights
Lab Chip, 2009, 9, 3463
DOI: 10.1039/b922116f
Petra Dittrich reviews the current literature in miniaturisation and related technologies.
Papers
PCR amplification and genetic analysis in a microwell cell culturing chip
Sara Lindström, Maria Hammond, Hjalmar Brismar, Helene Andersson-Svahn and Afshin Ahmadian,
Lab Chip, 2009, 9, 3465
DOI: 10.1039/b912596e
A methodology for single-cell culturing (i.e. clonal analysis), cell lysis, PCR amplification and mutation screening; all steps performed and detected on-chip in hundreds of individual microwells simultaneously. Heterogeneity in cell proliferation can be linked to the cells
genetic data, besides being a robust alternative to single-cell PCR in for example mutation frequency screenings.
Field-effect based attomole titrations in nanoconfinement
Rogier B. H. Veenhuis, Egbert J. van der Wouden, Jan W. van Nieuwkasteele, Albert van den Berg and Jan C. T. Eijkel,
Lab Chip, 2009, 9, 3472
DOI: 10.1039/b913384d
We demonstrate proton titration in a nanochannel by applying the field effect to release protons from the silicon nitride insulating channel walls. An amount of TRIS of only 100 attomol was detected using fluorescein as fluorescent pH indicator.
Room-temperature intermediate layer bonding for microfluidic devices
Jacob Bart, Roald Tiggelaar, Menglong Yang, Stefan Schlautmann, Han Zuilhof and Han Gardeniers,
Lab Chip, 2009, 9, 3481
DOI: 10.1039/b914270c
Microfluidic systems that constitute room-temperature intermediate layer bonds based on chemically activated fluorine ethylene propylene (FEP) sheets inserted between modified glass/silicon substrates can be operated at fluidic pressures up to 10 bar.
Print-n-Shrink
technology for the rapid production of microfluidic chips and protein microarrays
Kevin Sollier, Céline A. Mandon, Kevin A. Heyries, Loïc J. Blum and Christophe A. Marquette,
Lab Chip, 2009, 9, 3489
DOI: 10.1039/b913253h
Screen-printed microfluidic components and protein spots deposited at the surface of shrinkable polystyrene give rise to a complete biochip integrating both complex microfluidic designs and protein spots for bioanalytical applications.
A blood sampling microsystem for pharmacokinetic applications: Design, fabrication, and initial results
Tao Li, Adam Barnett, Karen L. Rogers and Yogesh B. Gianchandani,
Lab Chip, 2009, 9, 3495
DOI: 10.1039/b910508e
A blood sampling microsystem for laboratory animals in pharmacokinetic studies eliminates the need for tethers and catheters, and reduces the large dead and transfer volumes associated with conventional options.
Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles
D. M. Bruls, T. H. Evers, J. A. H. Kahlman, P. J. W. van Lankvelt, M. Ovsyanko, E. G. M. Pelssers, J. J. H. B. Schleipen, F. K. de Theije, C. A. Verschuren, T. van der Wijk, J. B. A. van Zon, W. U. Dittmer, A. H. J. Immink, J. H. Nieuwenhuis and M. W. J. Prins,
Lab Chip, 2009, 9, 3504
DOI: 10.1039/b913960e
We describe an integrated optomagnetic immunoassay biosensor technology based on nanoparticles that are magnetically actuated and optically detected in a stationary sample fluid.
High-pressure on-chip mechanical valves for thermoplastic microfluidic devices
Chien-Fu Chen, Jikun Liu, Chien-Cheng Chang and Don L. DeVoe,
Lab Chip, 2009, 9, 3511
DOI: 10.1039/b912014a
Discrete elastomer valves integrated into rigid thermoplastic chips are demonstrated, with high pressure resistance above 12 MPa over repeated open/close cycles without leakage. Proportional operation is demonstrated using a chemical gradient generator fabricated in a cyclic olefin polymer chip.
Correlating short-term Ca2+ responses with long-term protein expression after activation of single T cells
Michael Kirschbaum, Magnus Sebastian Jaeger and Claus Duschl,
Lab Chip, 2009, 9, 3517
DOI: 10.1039/b911865a
Cell signaling events on highly differing time scales are investigated on the level of individual cells. In a dielectrophoresis–microfluidics combination, we use T lymphocytes as a model system for surface contact-mediated cell activation.
2D-PCR: a method of mapping DNA in tissue sections
Michael Armani, Jaime Rodriguez-Canales, John Gillespie, Michael Tangrea, Heidi Erickson, Michael R. Emmert-Buck, Benjamin Shapiro and Elisabeth Smela,
Lab Chip, 2009, 9, 3526
DOI: 10.1039/b910807f
A multi-well device is paired with carefully designed chemistry to perform high-throughput DNA extraction, amplification, and detection in a parallel format, allowing for the localization of molecular targets across a tissue section.
Microfluorimeter with disposable polymer chip for detection of coeliac disease toxic gliadin
Teresa Mairal, Ines Frese, Enrique Llaudet, Carmen Bermudo Redondo, Ioanis Katakis, Frithjof von Germar, Klaus Drese and Ciara K. O' Sullivan,
Lab Chip, 2009, 9, 3535
DOI: 10.1039/b914635k
A microfluorimeter device for the in situ detection of gliadin in foodstuffs, which could be used for a rapid control of raw materials in food processing, as well as for process control of gliadin contamination, is described.
Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of care
Katherine G. McKenzie, Lisa K. Lafleur, Barry R. Lutz and Paul Yager,
Lab Chip, 2009, 9, 3543
DOI: 10.1039/b913806d
A method is described for rapid and specific protein depletion from human plasma using ligand immobilized beads stored dry within a novel, pneumatically-driven mixer as a part of sample pretreatment prior to analytical processing.
Detection of viruses with molecularly imprinted polymers integrated on a microfluidic biochip using contact-less dielectric microsensors
Gerald M. Birnbaumer, Peter A. Lieberzeit, Lukas Richter, Romana Schirhagl, Marcus Milnera, Franz L. Dickert, Andrew Bailey and Peter Ertl,
Lab Chip, 2009, 9, 3549
DOI: 10.1039/b914738a
Continuous detection of viral contamination using two native- and imprinted-polymers (MIPs) integrated into two microfluidic channel networks with contact-less bioimpedance spectroscopy.
Highly parallel introduction of nucleic acids into mammalian cells grown in microwell arrays
Tilak Jain, Ryan McBride, Steven Head and Enrique Saez,
Lab Chip, 2009, 9, 3557
DOI: 10.1039/b913794g
We have created microwell arrays on an electroporation-ready transparent substrate and established procedures to achieve highly efficient parallel introduction of exogenous molecules into cell lines and primary cells.
Sequential processing from cell lysis to protein assay on a chip enabling the optimization of an F1-ATPase single molecule assay condition
Tetsuya Nakayama, Moriaki Namura, Kazuhito V. Tabata, Hiroyuki Noji and Ryuji Yokokawa,
Lab Chip, 2009, 9, 3567
DOI: 10.1039/b911148d
We propose an integrated protein assay device, which includes cell lysis, protein extraction, purification, and activity assay at the single-molecule scale. This Single Molecule MicroTAS (SMM) provides an on-chip platform for mobility measurements of motor proteins (F1-ATPase) by injecting E. coli. The condition for the ATP-driven rotation assay was optimized by lysate diffusion in microchannels.
Multifunctional plasmonic sensors on low-cost subwavelength metallic nanoholes arrays
Valentin Canpean and Simion Astilean,
Lab Chip, 2009, 9, 3574
DOI: 10.1039/b914235e
Localized surface plasmon resonance (LSPR) sensing is combined with surface enhanced Raman scattering (SERS) detection on periodic arrays of subwavelength metallic nanoholes for the first time in a synergistic approach that opens a route for molecular dual-modal detection to be integrated in lab-on-chip systems to increase the reliability of biological detection.
Rapid multicomponent optical protein patterning
Jonathan M. Bélisle, Dario Kunik and Santiago Costantino,
Lab Chip, 2009, 9, 3580
DOI: 10.1039/b911967a
Widefield illumination LAPAP is a simple optical method to produce arbitrary substrate-bound protein pattern by photobleaching fluorescently conjugated molecules. Patterns can now be fabricated in a few minutes and we show two methods to obtain multicomponent protein gradients.
Micro contactor based on isotachophoretic sample transport
Gabriele Goet, Tobias Baier and Steffen Hardt,
Lab Chip, 2009, 9, 3586
DOI: 10.1039/b914466h
It is demonstrated how isotachophoresis (ITP) in a microfluidic device may be utilized to bring small sample volumes into contact in a well-controlled manner. This opens up the possibility of using such a device as a special type of micromixer with mixing times significantly below one second and an option to regulate the duration of contact.
Technical Notes
An automated, pre-programmed, multiplexed, hydraulic microvalve
Jitae Kim, Dafeng Chen and Haim H. Bau,
Lab Chip, 2009, 9, 3594
DOI: 10.1039/b914865e
Multiple hydraulic valves are actuated by line pressure controlled with rollers moving over a prefabricated topology.
Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms
Daniel Mark, Tobias Metz, Stefan Haeberle, Sascha Lutz, Jens Ducrée, Roland Zengerle and Felix von Stetten,
Lab Chip, 2009, 9, 3599
DOI: 10.1039/b914415c
We present a new centrifugal microfluidic valve that stops and forwards liquid by passively generated air counter-pressure. It allows robust metering also for highly wetting liquids.
Back matter
Lab Chip, 2009, 9, 3604
DOI: 10.1039/b923625m
Back cover
Lab Chip, 2009, 9, 3607
DOI: 10.1039/b923626k
RSS feed
