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- Legend
- Figures S1–S3: RNA cleavage kinetics for single C AON (5), double C AON (6) and DPPZ conjugated double C AON (9) in different RNA concentrations (0.008–1 µM) keeping the same AONs (5 µM) and RNase H concentration (0.06 U in 30 µl of reaction mixture)
- Figure S1
- Figure S2
- Figure S3
- Figures S4–S8: RNA cleavage kinetics for native 15mer AON (1), single T AON (2), double T AON (3) and triple T AON (4) in different RNA concentrations (0.01-3 µM) keeping the same AONs (5 µM) and RNase H concentration (0.06 U in 30 µl of reaction mixture). The kinetics of the control native 15mer AON (1) and double T AON (3) with 1 µM and RNA (14) with 0.1µM concentration, used for the calibration of the exact RNase H concentration, were also shown
- Figure S4
- Figure S5
- Figure S6
- Figure S7
- Figure S8
- Figures S9–S14: RNA cleavage kinetics for native 15mer AON (1), single T AON (2), double T AON (3) and triple T AON (4) in different RNA concentrations (0.01-3 µM) keeping the same AONs (5 µM) and RNase H concentration (0.12 U in 30 µl of reaction mixture). The kinetics of the control native 15mer AON (1) and double T AON (3) with 1 µM and RNA (14) with 0.1µM concentration, used for the calibration of the exact RNase H concentration, were also shown
- Figure S9
- Figure S10
- Figure S11
- Figure S12
- Figure S13
- Figure S14
- Figures S15–S23: RNA cleavage kinetics for native 15mer AON (1), 15-DPPZ AON (7), 15-3T-DPPZ AON (8), 15-3T-Cholest AON (10) and 15-2C-Cholest AON (11) in different RNA concentrations (0.01-1 µM) keeping the same AONs concentration (5 µM). The kinetics of the control native 15mer AON (1) with 1 µM and RNA (14) with 0.1µM concentrations, used for the calibration of the exact RNase H concentration, were also shown in Figure S15
- Figure S15
- Figure S16
- Figure S17
- Figure S18
- Figure S19
- Figure S20
- Figure S21
- Figure S22
- Figure S23