All aspects of the technology and system are covered - from an overview of a typical HPLC system and important considerations when combining this separation technique with detection by mass spectrometry, all the way through the various atmospheric ionisation processes and the different mass analysers that are available. The main focus is on fundamental concepts, but important practical aspects and some basic troubleshooting will also be introduced. All of which will have an impact on day to day analyses in the lab.
Session 1 | Runtime - 120 min.
Introduction to LC-MS and HPLC
Ionisation – APCI
Ionisation – APPI
Ionisation - ESI
Session 2 | Runtime - 114 min.
Quadrupole Mass Analysers
Triple Quadrupole Experiment Types
Time-of-Flight Mass Analysers
Ion Trap Mass Analysers
Magnetic Sector and Orbitraps
Mass Analyser Applications
Session 3 | Runtime - 78 min.
HPLC-MS Considerations
ESI HPLC Considerations
APCI HPLC Considerations
Introduction to the Detector
Vacuum Pumps
Analyte Enrichment and Vacuum Maintenance
Session 4 | Runtime - 78 min.
Resolution and Mass Accuracy
Interpretation
Method Development
Each video session is accompanied by supporting modules from CHROMacademy and we will use the animations within CHROMacademy to clearly illustrate the science and processes that are being discussed. All four sessions should be completed in order to gain the full training. The final assessment must be completed in order to gain your certificate of completion.
Course Delivery & Duration
• 4 video sessions each session is between 1-2 hours long
• Tutor supported with access to our chromatography experts
• Supporting training material
• Course assessment with certificate
Our CHROMacademy tutors will deliver four video training sessions which you can access on demand. They will provide online support and tutoring where necessary.
This course has been designed for anyone who is new to LC-MS, or those wishing to develop their practical understand of LC-MS analysis.
Additional infoSign up for 1-year CHROMacademy Premier Membership ($469.00 / £349.00 / €419.00 per year) and you get access to this course and FIVE other video courses - as well as hundreds of multi-media online modules in analytical science.
Online
8 hours
£349.00 GBP (plus VAT)
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