This course will detail the fundamental aspects that are required to fully understand HPLC. We will focus on the theory of HPLC and the fundamental mechanisms that result in chromatographic separation. We deliver practical insights into the decisions that are required to design robust mobile phases and to select the correct column for your application. Each component of the HPLC instrument will also be discussed, allowing for a better understanding of how the equipment works which in turn will aid in day to day good practice and troubleshooting.
The material covered in this course includes:
Session 1 | Runtime - 75 min.
Introduction to HPLC
Chromatographic Parameters
Reversed Phase HPLC - Introduction & Separation Mechanism
Reversed Phase HPLC - Solvents
Reversed Phase HPLC - Working with Ionizable Analytes
Session 2 | Runtime - 110 min.
HPLC Columns – Stationary Phases and Column Dimensions Explained
Isocratic and Gradient Elution
Session 3 | Runtime - 95 min.
Mobile Phase Considerations
Solvent Pumping Systems
Autosamplers
Session 4 | Runtime - 120 min.
Detectors
Basic Column & System Care & Maintenance
Quantitative and Qualitative HPLC
Each video session is accompanied by supporting modules from CHROMacademy and we will use the animations within CHROMacademy to clearly illustrate the science and processes that are being discussed. All four sessions should be completed in order to gain the full training. The final assessment must be completed in order to gain your certificate of completion.
Course Delivery & Duration
• 4 video sessions each session is between 1-2 hours long
• Tutor supported with access to our chromatography experts
• Supporting training material
• Course assessment with certificate
Our CHROMacademy tutors will deliver four video training sessions which you can access on demand. They will provide online support and tutoring where necessary.
This course has been designed for novice HPLC users and those who have limited experience of HPLC operation. Everyone is welcome!
Additional infoSign up for 1-year CHROMacademy Premier Membership ($469.00 / £349.00 / €419.00 per year) and you get access to this course and FIVE other video courses - as well as hundreds of multi-media online modules in Analytical Science
Online
8 hours
£349.00 GBP (plus VAT)
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