HPLC Troubleshooting

29 June 2009, London, United Kingdom

Introduction

HPLC Instrumentation
• An overview of the HPLC instrument
• The solvent
• The pump
• The injector
• Connections, tubing and fittings
• The detector
HPLC Columns
• Reversed phase column or stationary phase?
• Understanding interactions
• Care and maintenance
• Peaks, retention and temperature
The Liquid Chromatographic Process