HPLC Method Development & Optimisation

30 June 2009, London, United Kingdom

Introduction

Resolution
• Efficiency
• Capacity factor
• Selectivity
HPLC Columns
• The matrix
• The stationary phase
The Mobile Phase
• Elution types
• pH effects
• pH modifiers
• Ion pairing
• Temperature
Optimising LC Methods
• Isocratic separations
• Gradient separations
UHPLC: Investigations into technical developments