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EMBL Course - Microinjection in Zebrafish and Medaka: From Transgenesis to CRISPR
20 November 2018 09:00 - 22 November 2018 13:00, Heidelberg, Germany
Introduction
Microinjection into zebrafish and medaka embryos will be covered, as well as subsequent screening to identify positive founder fish.
Audience
This course is aimed at research technicians and scientists interested in the generation of genetic modified fish models. Participants should have basic experience with molecular biology techniques.
Modules/Resources
Microinjection of lineage tracers into one-cell stage embryos
Co-injection of DNA and mRNA into one-cell stage embryos
Microinjection of CRISPR/Cas constructs
Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
Homology directed repair in fish
Screening by imaging
High throughput, high content screening for successful events
Molecular validation of Crispr/Cas9 mediated indel formation
Learning Outcomes
The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.
