Complete HPLC & and LC-MS
What will you learn on this course?
The Complete HPLC & LC-MS course is built from the Practical Essentials of HPLC & LC-MS and the HPLC & LC-MS Clinic courses to provide a complete training solution enabling you to understand your Liquid Chromatograph or LC-MS instrument, application method development, troubleshooting & maintenance.This course covers all the practical theory you need to know about liquid chromatography and its related techniques including liquid chromatography-mass spectrometry. Mobile phase optimisation including gradient elution, pumps and plumbing, autosampler injection techniques, sample preparation, calibration standards, data analysis for quantitation and qualitative analysis are discussed.
It also looks at how to develop appropriate application specific HPLC & LC-MS methods and the set-up and optimisation of instrumentation. Finally the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered. Advanced techniques are discussed and their application to difficult samples requiring optimal limits of detection and linear dynamic range. HPLC to UHPLC method transfer is touched upon, chromatographic deconvolution utilising tandem mass spectrometry, multi-dimensional LC (LCxLC), on-line SPE and chemometrics.
Learning outcomes:
- You will learn the importance of good chromatography
- You will learn how to select an appropriate mobile phase and develop gradient elution methods
- You will learn about various autosampler injection techniques and when to use them
- You will learn how to select an HPLC column (stationary phase and dimensions)
- You will learn the operation and selection of an appropriate HPLC detector
- You will learn about the principles and components of the mass spectrometer
- You will learn about sample preparation, calibration standards and sample properties
- You will learn how to undertake data analysis for quantitation and qualitatitive analysis
- You will learn strategies for HPLC and LC-MS method development
- You will learn about system optimisation and configuration for HPLC and LC-MS
- You will get an introduction to LC & MS advanced techniques
- You will recognise areas requiring maintenance on the instrument
- You will learn the logical steps of instrument troubleshooting
This course has been approved by the Royal Society of Chemistry for purposes of Continuing Professional Development (CPD).
Find out more and book online or email: courses@anthias.co.uk
