File Name : fig.s1.tif

Caption : fig.s1. t<sub>max</sub> and t<sub>min</sub> optimization of asea for cpv detection. (a) t<sub>max</sub> optimization at t<sub>min</sub> of 61 ℃. asea reactions were carried out at the t<sub>max</sub> of (1) 75 ℃, (2) 74 ℃, (3) 73 ℃, (4) 72 ℃ and (5) 71 ℃, respectively. 1’-5’ represented corresponding ntc. (b) t<sub>min</sub> optimization at the t<sub>max</sub> of 72 ℃. asea reactions were carried out at the t<sub>min</sub> of (1) 63 ℃, (2) 62 ℃, (3) 61 ℃, (4) 60 ℃ and (5) 59 ℃, respectively. 1’-5’ represented corresponding ntc. the cpv genomic dna at the concentration of 1.0×10<sup>-13</sup> m was employed for thermal cycling optimization.

File Name : fig.s2.tif

Caption : fig.s2. the fluorescence curves of asea reactions with the template of cpv genomic dna at the concentration of (1) 1.0×10<sup>-17</sup> m, (2) 8.0×10<sup>-18</sup> m, (3) 6.0×10<sup>-18</sup> m, (4) 4.0×10<sup>-18</sup> m, (5) 2.0×10<sup>-18</sup> m, and (6) 1.0×10<sup>-18</sup> m. ntc represented no target control.