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Caption : supplementary figure s1 pfos effectively induces tm4 cell injury. (a) cellular viability. tm4 cells were seeded in 96-well plates at a concentration of 3 × 104 cells/well and treated with serial concentrations of pfos (10-3–103 µm) for 24 h. (b-d) tm4 cells were seeded in 6-well plates at a density of 5 × 104/well and treated with various concentrations of pfos (25, 50, 100, 150 µm) for 24 h. (b) representative images of cell morphology (scale bar = 25 μm). (c) the relative expression levels of zo-1, occludin and claudin-11. (d) the relative expression levels of cx43 and p-cx43. *p < 0.05, **p < 0.01 compared with control. 

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Caption : supplementary figure s2 pfos causes sertoli cell injury by upregulating p38mapk/mmp9 pathway. the relative expression levels of p38mapk, p-p38mapk and mmp9 were detected using western blot analysis. *p < 0.05, **p < 0.01 compared with control.