File Name : schematic s1.jpeg

Caption : schematic s1: treatment schedule of the bioreactors. three bioreactors (v1-3) were inoculated with a faecal microbiome on day 0. following a 10-day equilibration period/steady-state phase (referred to hereafter as the pre-supplementation phase), feso4 (10 mm) containing sodium ascorbate (50 mm) was introduced into the vessels to evolve the iron concentration within to 100 µm over three consecutive days. thereafter, the iron concentration within the bioreactors was maintained at 100 µm (referred to hereafter as the iron-supplementation phase).  following this, lignin was also co-administered (with iron) by injecting an aqueous lignin solution to maintain bioreactor lignin concentrations at 0.3 % (w/v) in each vessel (referred to hereafter as the lignin + iron supplementation phase). samples were taken throughout the experimental phases for iron analysis. for microbial community changes, samples were obtained at two time points at the end of each of the three phases (for example, day 8 and day 10 in each 10-day experimental phase period).