File Name : figure s1-submit.tif
Caption : fig. s1 selection of the secondary structures of t.vag-1 to t.vag-10. in the 10 sets of primers for t. vaginalis, t.vag-3 and t.vag-6 produced all negative results within 60 min in 8 independent replications, respectively, and were selected to step 2. rfs, relative fluorescence signal.
File Name : figure s2-submit.tif
Caption : fig. s2 selection of the sensitivity of t.vag-3 and t.vag-6. t.vag-6 produced all positive results within 40 min in 3 independent replications, when the concentration of template was low to 102 copies/reaction, better than t.vag-3, thus was selected to step 3. rfs, relative fluorescence signal; nc, negative control, using deionized water as the template; 10^1-10^3 (i.e. 101-103), the concentration of the template (copies/reaction).
File Name : figure s3-submit.tif
Caption : fig. s3 validation of the sensitivity of t.vag-6. t.vag-6 produced all positive results within 40 min in 20 independent replications, when the concentration of template was 102 copies/reaction, thus was selected to step 4. rfs, relative fluorescence signal; nc, negative control, using deionized water as the template; 10^2 (i.e. 102), the concentration of the template (copies/reaction).
File Name : figure s4-submit.tif
Caption : fig. s4 validation of the secondary structure of t.vag-6. t.vag-6 produced negative results within 50 min in 89 independent replications, and was selected to step 5. rfs, relative fluorescence signal; nc, negative control, using deionized water as the template; 10^4 (i.e. 104), positive control with the targeted template of 104 copies/reaction.
File Name : figure s5-submit.tif
Caption : fig. s5 validation of the specificity of t.vag-6. t.vag-6 produced positive results only with the targeted template within 40 min in 3 independent replications, when the concentration of all templates was 105 copies/reaction, and was selected to detect t. vaginalis in our study. rfs, relative fluorescence signal; nc, negative control, using deionized water as the template.