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Caption : supplementary figure 1. immunofluorescence staining of  oct-4 (green), actin myofibrils (red) of dfats, and hoechst counterstaining was used to visualize nuclei (blue) of dfats. the colocalization of oct-4, f-actin and nuclei of dfats are shown in merge image (scale bars: 50 µm).


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Caption : supplementary figure 2. f-actin staining of dfats cultured on pll coated and uncoated surfaces. hoechst counterstaining was used to visualize nuclei (blue) of dfats . (scale bar: 50 µm).


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Caption : supplementary figure 3. representative flow cytometry histograms of dedifferentiated fat cells (dfats) obtained from uncoated surface at passage 4 (n=3).



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Caption : supplementary figure 4. multilineage differentiation of dfats. osteogenic differentiation indicated by alizarin red s staining on day 21 (left),  chondrogenic differentiation indicated by alcian blue staining on day 14 (middle), adipogenic differentiation indicated by oil red o staining on day 14 (right) (scale bar: 100 µm).



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Caption : supplementary figure 5. protein concentration of dmem included 0, 10 and 20% fbs (v/v). statistically significant differences are stated by symbols:  ***p<0.0001 when the control group is dmem included 0% fbs (v/v), n=3.


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Caption : supplementary figure 6. cell viability analysis of dfats on day 7. a) representative clsm images of live/dead staining on a) pah coated ps surface.  b) uncoated ps surface (scale bars: 100 µm). c) cell viability percentages of dfats on uncoated and pah coated surfaces . statistically significant differences are stated by symbols:  ***p<0.001 when the control group is living cells from uncoated surface, •••p<0.001 when the control group is dead cells from uncoated surface, n=5.


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Caption : supplementary figure 7. phase contrast images of mature adipocytes ceiling culture with a) gelatin, b) gellan gum coated polystyrene surfaces. (scale bar: 100 µm).


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Caption : supplementary figure 8. fluorescence labelled polymer coating on tissue culture polystyrene well plates. a) representative clsm images on day 0, and day 7 (n=3, scale bar: 1mm),  b) fluorescence intensity values of fluorescence labelled polymer coated surfaces.  c) remaining fluorescence intensity of polymer coated surfaces on day 7. (col i: collagen i, col iv: collagen iv, lam: laminin, fib: fibronectin, pll: poly-l-lysine, gel: gelatin, gg: gellan gum, fga: fluoresceinyl glycine amide, fitc: fluorescein isothiocyanate, fam: fluorescein amidite, rho: rhodamine, n.s.: not significant). statistically significant differences are stated by symbols: *p<0.1 and **p<0.01 when the control group is fi of coated surfaces on day 0, n=3.