File Name : fig.s1.tif Caption : uv-vis absorbance of rgo nanoflake. File Name : fig.s2.tif Caption : micro raman spectra for rgo mixed pdms thin sheet device File Name : fig.s3.tif Caption : afm image and histogram at a surface area of (a) 3 µm2 (b) 10 µm2 (c) 25 µm2 (d) 50 µm2 File Name : fig.s4.tif Caption : control experiment using pi dye delivery nih3t3 cells. images captured after performing photoporation without rgo mixed pdms sheet device (a-d) and with device(e-f). File Name : fig.s5.tif Caption : : rgo concentration optimization studies on l929 cells. images captured after performing photoporation with different concentrations (0.3 mg/ml, 0.8 mg/ml, 1.3 mg/ml, 1.6 mg/ml). File Name : fig.s6.tif Caption : scanning speed optimization studies. images captured after performing photoporation in l929 cells with different scanning speed (10 mm/s, 9 mm/s, 6 mm/s) File Name : fig.s7.tif Caption : flow cytometry data for the dextran 3000 delivery using rgo mixed pdms device. (a) shows total cell count, out of which (b) shows percentage of live cells in the sample, (c) shows percentage of cells delivered with dextran, and (d) shows percentage of viable cells which are delivered. File Name : fig.s8.tif Caption : : control experiment for pi dye delivery in l929 cells. (a-c) without laser exposed on cells without a device with biomolecules, confirm no pi dye delivery (red), calcein am (green) shows live cell image and merge image confirms most the cells are live (d-f) laser esposed on cells without a device and with biomolecules, which confirms no pi dye delivery (red), calcein am corresponding live cells and the merged image confirmed all cells are live (g-i)laser exposed on cells with a device with biomolecules confirm pi dye delivery (red), calcein am image (green) confirm the live cell and merge image (green to yellowish green) confirm the live cell after pi dye delivery. File Name : fig.s9.tif Caption : control experiment for pi dye delivery nih3t3 in cells. (a-c) without laser exposed on a device with biomolecules, confirm no pi dye delivery (red), calcein am (green) shows live cell image and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with biomolecules, which confirms no pi dye delivery (red), calcein am corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm pi dye delivery (red), calcein am image (green) confirm the live cell and merge image (green to yellowish green) confirm the live cell after pi dye delivery. File Name : fig.s10.tif Caption : : control experiment using dextran 3000 da delivery in nih3t3 cells. cells exposed to laser after adding biomolecules without incorporating rgo mixed pdms sheet device (a-d), and cells exposed to laser after adding biomolecules with rgo mixed pdms sheet device placed on top of cells (e-h). File Name : fig.s11.tif Caption : control experiment for 3kda dextran delivery in nih3t3 cells. (a-c) without laser exposed on a device with biomolecules, confirm no dextran delivery (red), calcein am (green) shows live cell image and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with biomolecules, which confirms no pi dye delivery (red), calcein am corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm dextran delivery (red), calcein am image (green) confirm the live cell and merge image (green to yellowish green) confirm the live cell after dextran delivery. File Name : fig.s12.tif Caption : control experiment for sirna delivery in hela cells. (a-c) without laser exposed on a device with biomolecules, confirm no sirna delivery (green), calcein image (red) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with sirna biomolecules, which confirms no sirna delivery (green), calcein image (red) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm sirna delivery (green), calcein image (red) confirm the live cell and merge image (green to yellowish green) confirm the live cell after sirna delivery. File Name : fig.s13.tif Caption : control experiment for sirna delivery in n2a cells(a-c) without laser exposed on a device with biomolecules, confirm no sirna delivery (green), calcein image (red) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with sirna biomolecules, which confirms no sirna delivery (green), calcein image (red) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm sirna delivery (green), calcein image (red) confirm the live cell and merge image (green to yellowish green) confirm the live cell after sirna delivery. File Name : fig.s14.tif Caption : : control experiment for sirna delivery in nih3t3 cells. (a-c) without laser exposed on a device with biomolecules, confirm no sirna delivery (green), calcein image (red) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with sirna biomolecules, which confirms no sirna delivery (green), calcein image (red) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm sirna delivery (green), calcein image (red) confirm the live cell and merge image (green to yellowish green) confirm the live cell after sirna delivery. File Name : fig.s15.tif Caption : : control experiment for delivery of plasmid for egfp expression in huh7 images. cells exposed to laser after adding biomolecules without incorporating rgo mixed pdms sheet device (a-d), and cells exposed to laser after adding biomolecules with rgo mixed pdms sheet device placed on top of cells (e-h). File Name : fig.s16.tif Caption : control experiment for delivery of plasmid for egfp expression in hela cells. (a-c) without laser exposed on a device with biomolecules, confirm no plasmid for egfp delivery (green), calcein image (red) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with egfp biomolecules, which confirms no egfp delivery (green), calcein image (red) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm egfp delivery (green), calcein image (red) confirm the live cell and merge image (green to yellowish green) confirm the live cell after egfp delivery. File Name : fig.s17.tif Caption : control experiment for enzyme delivery in huh7 cells. cells exposed to laser after adding biomolecules without incorporating rgo mixed pdms sheet device (a-c), and cells exposed to laser after adding biomolecules with rgo mixed pdms sheet device placed on top of cells (d-f). File Name : fig.s18.tif Caption : control experiment for enzyme delivery in l929 cells. (a-c) without laser exposed on a device with enzyme biomolecules, confirm no enzyme delivery (red), calcein am image (green) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with biomolecules, which confirms no enzyme delivery (red), calcein image (green) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm enzyme delivery (red), calcein image (green) confirm the live cell and merge image (green to yellowish green) confirm the live cell after enzyme delivery. File Name : fig.s19.tif Caption : control experiment for enzyme delivery in nih3t3 cells. (a-c) without laser exposed on a device with enzyme biomolecules, confirm no enzyme delivery (red), calcein am image (green) shows live cell and merge image confirms most the cells are live (d-f) laser exposed on cells without a device and with biomolecules, which confirms no enzyme delivery (red), calcein image (green) corresponding live cells and the merged image confirmed all cells are live (g-i) laser exposed on a device with biomolecules confirm enzyme delivery (red), calcein image (green) confirm the live cell and merge image (green to yellowish green) confirm the live cell after enzyme delivery. File Name : fig.s20.tif Caption : cytotoxicity studies. 3 day mtt assay performed on cells which haven’t undergone optoporation(control) and cells which underwent optoporation using our platform(device).