Modern analytical methods and production processes (manufacturing and research) are complex, with many different factors affecting the outcome. In order to be competitive, companies need to minimize resources expended on development and maximize process performances. Design of Experiments (DoE) enables these complex situations to be understood, reducing the cost of gaining an in-depth knowledge of the process which can be translated into competitive advantage. It is also a tool required by most or the regulatory agencies worldwide (including, for example, US Food and Drug Administration). DoE provides a well structured method for determining the relationships between factors affecting the process under study.