Basic Concepts of HPLC
This short seminar is intended as a first introduction to ‘HPLC’ and is suitable for anyone who wants to gain a basic understanding of this technique. The basis of the separation process and retention mechanisms will be covered, as well as how the important parameters of resolution, peak shape and efficiency are measured. An outline of HPLC hardware and different modes of separation will be considered. This course will provide a good foundation from which to progress to more advanced training. A number of topics will be covered during the seminar:-
Principles and history of chromatography
Basics of the chromatographic process
HPLC column chemistry
Components of an HPLC system
Important chromatographic parameters
HPLC separation modes
This short seminar is intended as a first introduction to ‘HPLC’ and is suitable for anyone who wants to gain a basic understanding of this technique. The basis of the separation process and retention mechanisms will be covered, as well as how the important parameters of resolution, peak shape and efficiency are measured. An outline of HPLC hardware and different modes of separation will be considered. This course will provide a good foundation from which to progress to more advanced training. A number of topics will be covered during the seminar:-
Principles and history of chromatography
Basics of the chromatographic process
HPLC column chemistry
Components of an HPLC system
Important chromatographic parameters
HPLC separation modes
