Who should take this course?
This course is designed for anyone who works with HPLC or UHPLC. No previous experience with
HPLC or UHPLC systems is assumed; however, much of the course will also appeal to the more
experienced chromatographer.
What does it cover?
“HPLC & UHPLC Troubleshooting” is an intensive one day course that teaches you how to solve
the common problems that can occur with your LC methods. You will learn how to qualify the
performance of your HPLC/UHPLC instrument using specific tests that can also be used for
isolating existing problems. You’ll also learn how to prevent many of these problems from occurring
in the first place. This course covers:
• Operating principles of each module in an HPLC/UHPLC system
• Review of HPLC theory as it applies to troubleshooting and instrument maintenance
• Why performance qualification (PQ) is so important
• Proven techniques for systematic problem solving and instrument maintenance
• The most effective, time saving and money saving approaches to preventing common
hardware problems and method failures
What will I get from this course?
What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating
problems you experience have straightforward and logical solutions. More importantly, you’ll learn
that most of these problems are preventable. The techniques you learn from this course will make
you more effective at work and save you big headaches! Delegates tell us that this “extremely
practical course” is a must for everyone who uses HPLC or UHPLC.
Course Outline
Principles of HPLC & UHPLC troubleshooting
Performance qualification, part 1: pump & detector
PQ, part 2: on-line mixing
PQ, part 3: chromatographic checks
The separation: physical problems with columns
The separation: chemical problems with columns
Problems with quantification
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
This course is designed for anyone who works with HPLC or UHPLC. No previous experience with
HPLC or UHPLC systems is assumed; however, much of the course will also appeal to the more
experienced chromatographer.
What does it cover?
“HPLC & UHPLC Troubleshooting” is an intensive one day course that teaches you how to solve
the common problems that can occur with your LC methods. You will learn how to qualify the
performance of your HPLC/UHPLC instrument using specific tests that can also be used for
isolating existing problems. You’ll also learn how to prevent many of these problems from occurring
in the first place. This course covers:
• Operating principles of each module in an HPLC/UHPLC system
• Review of HPLC theory as it applies to troubleshooting and instrument maintenance
• Why performance qualification (PQ) is so important
• Proven techniques for systematic problem solving and instrument maintenance
• The most effective, time saving and money saving approaches to preventing common
hardware problems and method failures
What will I get from this course?
What you learn will demystify your instrument. You’ll find that all of the perplexing and frustrating
problems you experience have straightforward and logical solutions. More importantly, you’ll learn
that most of these problems are preventable. The techniques you learn from this course will make
you more effective at work and save you big headaches! Delegates tell us that this “extremely
practical course” is a must for everyone who uses HPLC or UHPLC.
Course Outline
Principles of HPLC & UHPLC troubleshooting
Performance qualification, part 1: pump & detector
PQ, part 2: on-line mixing
PQ, part 3: chromatographic checks
The separation: physical problems with columns
The separation: chemical problems with columns
Problems with quantification
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
