Introduction

Exploring the Power of Eluent pH in LC Method Development 
Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in analyte retention from eluent pH can be helpful for screening systems and method development platforms where observing the number of components in samples is important before method optimisation is attempted. The chromatographic selectivity 'space' for various eluent and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included. 
Exploring chromatographic selectivity
Stationary phase design
Acidic and basic analyte ionisation theory
Choosing appropriate stationary phases
HPLC and UHPLC
Practical hints and tips  

Available as in-house or off-site courses. 

Deadlines

• Mar 01 2018
  Early bird registration deadline

  
• Jul 03 2018
  Standard registration deadline

  

Useful links

• Further event details More information about the event

Speakers

• Helen Poole Hichrom Limited, United Kingdom

  http://www.hichrom.com
• Gemma Lo Hichrom Limited, United Kingdom

  http://www.hichrom.com

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Venue

Trinity Centre

Suite 1, Trinity Centre, 24 Cambridge Science Park, Milton Road, Cambridge, CB4 OFN, United Kingdom

Useful links

• Venue information More information about the venue

Organised by

Hichrom Ltd

Contact information

Jenny Sumner
Hichrom Limited
1 The Markham Centre
Station Road
Theale
Reading
Berkshire
RG7 4PE
01189303660
Contact us by email

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