Introduction

Exploring the Power of Eluent pH in LC Method Development 
Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in analyte retention from eluent pH can be helpful for screening systems and method development platforms where observing the number of components in samples is important before method optimisation is attempted. The chromatographic selectivity 'space' for various eluent and column permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical hints and tips for ensuring repeatable and reproducible chromatography, when working with an extended pH range, will be included. 
Exploring chromatographic selectivity
Stationary phase design
Acidic and basic analyte ionisation theory
Choosing appropriate stationary phases
HPLC and UHPLC
Practical hints and tips  
Available as in-house or off-site courses. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
 

Deadlines

• Mar 01 2018
  Early bird registration deadline

  
• Sep 24 2018
  Standard registration deadline

  

Speakers

• Dr Annette Larder Hichrom Limited, United Kingdom

  http://www.hichrom.com
• Gemma Lo Hichrom Limited, United Kingdom

  http://www.hichrom.com

---

Venue

TBC

TBC, TBC, TBC, Farnborough, ---, United Kingdom

Organised by

Hichrom Limited

Contact information

Jenny Sumner
Hichrom Limited
1 The Markham Centre
Station Road
Theale
Reading
Berkshire
RG7 4PE
01189303660
Contact us by email

---