Megan Wright (Astbury Centre for Structural Molecular Biology, School of Chemistry, University of Leeds)
Title: Design of covalent chemical probes to label proteins in living cells
Abstract: Chemical probes are powerful tools for dissecting biological mechanism in living systems and exploring drug targets. In my lab we are developing probes to label proteins in their native cellular environment. For example we design specific probes to label proteins of interest to image and study their function in cells, and elaborate bioactive molecules into probes with covalent or photocrosslinking groups to discover their protein targets via chemical proteomic workflows. In this talk I will focus on our development of a peptidic photoaffinity probe to label a human receptor that senses bacterial pathogens and host inflammation.
Antonio Angelastro (Manchester Institute of Biotechnology, University of Manchester & AstraZeneca)
Title: Synergising Biocatalysis and Synthetic Organic Chemistry for Site-Specific Modification of Native Proteins and Glycoproteins
Abstract: Proteins serve as the foundation of biology, playing crucial roles in cell structure, function, and regulation. Their chemical modification (e.g. bioconjugation) is fundamental for the advancement of biological sciences, from understanding the basis of cellular biology to biotherapeutics development. This virtual seminar aims to highlight the intersection of biocatalysis and synthetic organic chemistry for the site-specific modification of proteins and glycoproteins. By combining the strengths of both fields, highly specific and sophisticated modifications can be achieved, including native antibody olefination, with potential applications in biopharmaceuticals development, biotechnology, and materials science.
Title: Design of covalent chemical probes to label proteins in living cells
Abstract: Chemical probes are powerful tools for dissecting biological mechanism in living systems and exploring drug targets. In my lab we are developing probes to label proteins in their native cellular environment. For example we design specific probes to label proteins of interest to image and study their function in cells, and elaborate bioactive molecules into probes with covalent or photocrosslinking groups to discover their protein targets via chemical proteomic workflows. In this talk I will focus on our development of a peptidic photoaffinity probe to label a human receptor that senses bacterial pathogens and host inflammation.
Antonio Angelastro (Manchester Institute of Biotechnology, University of Manchester & AstraZeneca)
Title: Synergising Biocatalysis and Synthetic Organic Chemistry for Site-Specific Modification of Native Proteins and Glycoproteins
Abstract: Proteins serve as the foundation of biology, playing crucial roles in cell structure, function, and regulation. Their chemical modification (e.g. bioconjugation) is fundamental for the advancement of biological sciences, from understanding the basis of cellular biology to biotherapeutics development. This virtual seminar aims to highlight the intersection of biocatalysis and synthetic organic chemistry for the site-specific modification of proteins and glycoproteins. By combining the strengths of both fields, highly specific and sophisticated modifications can be achieved, including native antibody olefination, with potential applications in biopharmaceuticals development, biotechnology, and materials science.
