REAL TIME POLYMERASE CHAIN REACTION(RT-PCR) FOR IDENTIFICATION OF MICRO-ORGANISM

3 July 2023 09:00 - 7 July 2023 16:30, Nairobi, Kenya

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Introduction

Showing relative quantification of gene expression, quantification models, amplification
Standard operating procedure for PCR system,requirements of lab setup, instruments and software, dispensable and non-dispensable controls.
Basics of data analysis such as amplification plots, how to obtain Cq values, and melting curves analysis demonstration
Assay design and validation –reagents, probes and unspecific dyes, repeats, SNPs, secondary structures, specificity