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Hands-on training on enzyme-linked immunosorbent assay (ELISA) technique and PCR

13 May 2024 09:00 - 17 May 2024 16:00, KISUMU, Kenya


Introduction
HANDS-ON TRAINING ON ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA)TECHNIQUE                                              AND PCR (ELISA-PCR) 
DATE : 13th -17th MAY 2024
REGISTRATION DATE : 26th APRIL 2024
VENUE : WEST KENYA CANCER CARE RESEARCH CENTRE (WKCCRC ),KISUMU
COST : KSH.92800  OR  USD 928.00

COURSE OVERVIEW
Chrom Africa offers an outstanding platform for training on Polymerase Chain Reaction- Enzymes-linked Immunosorbent assay(PCR-ELISA) which is a commonly used analytical and biochemistry assay and or diagnostic tool in medicine,plant pathology,biotechnology and quality control check in various industries

  OBJECTIVES OF CONDUCTING HANDS-ON PCR-ELISA TRAINING
1.Understanding PCR-ELISA:
  • Participants will gain a comprehensive understanding of PCR-ELISA, which combines two powerful techniques: polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).
  • They will learn how to detect and quantify specific PCR products directly after immobilizing DNA on a microtiter plate.
2.Applications Across Fields:
  • Pathogen Detection: Trainees will explore the use of PCR-ELISA in diagnosing viruses in clinical samples (e.g., blood, serum, biopsies).
  • Bacterial and Fungal Identification: Accurate diagnosis of pathogenic bacteria and fungi to species level for appropriate treatment.
  • Genotyping: Understanding how to identify carriers of recessive diseases.
  • Environmental Monitoring: Analyzing fungal and bacterial isolates in hospitals.
  • Safety Assurance: Rapid monitoring of pathogens (e.g., salmonella) in dairy products.
  • Blood Donation Screening: Detecting contamination in blood products (e.g., hepatitis B antigens, HIV-specific IgG).
  • Epidemiology: Tracking the spread of pathogenic isolates and emergence of new strains.

ADVANTAGES OVER CONVENTIONAL ELISA 
PCR-ELISA offers several advantages:
  • Broad Target Range: It can detect genomic material from any biological specimen using suitable primers.
  • Less Dependency on Antibodies: Unlike conventional ELISA, it doesn’t require purified monoclonal antibodies and antigens.
  • Versatility: Applicable to various fields beyond pathogen detection1.
In summary, hands-on PCR-ELISA training equips participants with practical skills to confidently apply this hybrid technique in their research and diagnostic endeavors

SUITABILITY
This course is suitable for researchers, scientists, laboratory analysts, graduate students and postgraduate students who have a background in molecular biology/biotechnology,analytical chemistry or biochemistry, who are interested in learning more about the practical aspects of the course.
Venue
WEST KENYA CANCER CARE RESEARCH CENTRE (WKCCRC)

LABORATORY, WEST KENYA CANCER CARE RESEARCH CENTRE (WKCCRC), KISUMU-KAKAMEGA ROAD, , JARAMOGI OGINGA ODINGA TEACHING AND REFERAL HOSPITAL, KISUMU, 40100, Kenya

Organised by
CHROM AFRICA INSTRUMENTATION SERVICES LIMITED
Contact information
JOHN DONALD ANGIRA
CHROM AFRICA INSTRUMENTATION SERVICES LIMITED
BURUBURU BUSINESS COMPLEX SUITE NO.26, MUMIAS SOUTH ROAD, NAIROBI,P.O.BOX 4963 - 00100 NAIROBI, KENYA
+254719536664
Contact us by email

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