Applied Interpretation of LC-MS Mass Spectra

Introduction

This course is an introduction to mass spectral interpretation, providing essential tools and guidelines for analyzing full-scan LC-MS data. Participants will gain a deeper understanding of interpreting mass spectra generated by Electrospray Ionisation (ESI) and Atmospheric Pressure Chemical Ionisation (APCI).

Learning outcomes

You will learn the fundamentals of Electrospray Ionisation (ESI) and Atmospheric Pressure Chemical Ionisation (APCI) mass spectral interpretation for LC-MS
You will learn how to obtain high quality mass spectra and its importance
You will learn how electron ionisation occurs and review isotopes and isomers
You will learn simple techniques to recognise and elucidate spectra
You will learn how to observe fragmentation patterns for low molecular and high molecular weight molecules
You will learn about the formation of common adduct ions under different solvent and buffer conditions
You will learn how to use advanced tools to interpret spectra such as molecular ion, nitrogen rules, degree of unsaturation and losses
You will learn the importance of alpha and inductive cleavages and rearrangements

This course is taught live in a virtual classroom and offers a fully interactive experience, with instrument parts and consumables shown and explained. Participants can ask live questions; either by using their microphone or by typing into the chat box.

Royal Society of Chemistry members receive a discount of 15% for this course (membership number required)
CAMS members receive a discount of 15% for this course (membership name required)